Proteolytic mutants obtained from Clostridium botulinum type E.

Isolation of Clostridium botulinum type E from Cayuga Lake fish.

The vast majority of the isolations of Clostridium botulinum type E have been from marine fish and their environment, with very few from fresh-water lakes and streams. Johannsen (J. Appl. Bacteriol. 26:43, 1963) has found the organisms to be common in Norway and Sweden's coastal and river waters where the salinity is fairly low, and Prev6t and Huet (Bull. Acad. Natl. Med., Paris 135:432, 1951) ...

Cloning, Expression and Purification of Clostridium botulinum Neurotoxin Type E Binding Domain

Double Sandwich ELISA Modified Method for the Detection of Clostridium Botulinum Type E

Background & Objective: A very small amount of botulinum toxin can cause death and on the other hand, there is no cure for its poison other than antitoxin. Therefore, a diagnostic method that can detect very small amounts of botulinum toxin in a short time is very important. In this study, rapid and accurate detection of botulinum toxin type E has been performed with the double sandwich ELISA m...

Activation phenomenon of Clostridium botulinum type E toxin.

Gerwing, Julia (The University of British Columbia, Vancouver, Canada), Claude E. Dolman, and David A. Arnott. Activation phenomenon of Clostridium botulinum type E toxin. J. Bacteriol. 84:302-306. 1962-Highly purified preparations of both nonactivated and trypsin-activated type E botulinus toxins have been analyzed in an ultracentrifuge. Untreated botulinus type E toxin was found to have a sed...

Significance of 12S toxin of Clostridium botulinum type E.

The pathogenesis of type E botulism is discussed as an aspect of the physicochemical and biological properties of 12S toxins (prototoxin and trypsin-activated 12S toxin) and the Ealpha and Ebeta components of each 12S toxin. A molecular weight of 350,000 was determined for each 12S toxin and 150,000 for Ealpha and Ebeta. Owing to the structure comprising the subunits Ealpha and Ebeta, 12S toxin...